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Flow Cytometry and Flow Cytometry Training

Flow cytometry


Flow cytometry is a very powerful technology widely used for measuring the different characteristics of cells and particles. This technology has also advanced so rapidly from large space occupying flow cytometer to small compact table top instruments. During this advancement, the number of parameters that could be measured on a cell has also increased many folds and the cost has also come down considerably and become affordable to even small research and diagnostic laboratories. The major challenge is lack of trained people with required skill set to design a flow cytometry experiment, operate a flow cytometer, and analyze the flow data. To impart the skill set on flow cytometry to students, faculties, and any professionals, IBIOA has designed two hands on training programs: one on the basic flow cytometry for the beginners and another one on the advanced clinical flow cytometry training for the clinical pathologists, pathology residents, and clinical laboratory technicians with special emphasis on lymphoma and leukemia diagnosis.


Flow cytometry is a highly useful technique generally used for the measurement of any cell (mammalian, plant, algae, yeast, bacteria, etc) or particle (nuclei, chromosome, beads, nanoparticles, etc) while they are in a flowing stream. The light is focused on the cell in flow stream and then the scattered light and fluorescence of different wavelength are measured.  In the field of biology, flow cytometry is very widely used to study the blood cells, cancer cells, stem cells, etc. Flow cytometer is also used in the study of cell cycle, apoptosis, cytotoxicity, expressed proteins, nanoparticles, protein-protein interaction, and cell-cell interaction. The major advantage of flow cytometry is the ability to analyze many parameters of large number of different subsets of cells in a short period of time. The major disadvantage of flow cytometry is the absolute requirement of cells in single cell suspension, which in turn requires disruption of tissue architecture and their spatial arrangement.


Flow cytometry training


Flow cytometry training is very important to anyone who would like to use the flow cytometer for their research or diagnostic purposes. Flow cytometer is a complex high-end expensive instrument that requires proper training to design the experiment, prepare the samples, operate the instrument, and analyze the data. Flow cytometer training helps the person to understand the basic concepts behind the flow cytometry and impart skills to operate the flow cytometer instrument, and analyze the data in the right context. Flow cytometer components include light source, flow chamber, optical system, light detectors, computer, and software. Operating the flow cytometer without proper training might lead to breakdown of the machine, long downtime to repair, high cost to repair, delay in the project, and several other issues that could be avoided easily with proper training and understanding of flow cytometry. Flow cytometry training also helps in building someone’s career where a well-trained flow cytometer operator can become a flow cytometer facility manager in any academic or diagnostic laboratories or an outstanding cell biologist or clinical pathologist. The flow cytometry training we developed at IBIOA is in consultation with world renowned experts in flow cytometry trainings including Drs. VivekTanavde, Krishnamoorthy who are part of International society for flow cytometry as well as Indian flow cytometry society. Our basic flow cytometry training module is focused on the essential skill set required for using any flow cytometer and the advanced clinical flow cytometry training is focused on the specific immunophenotyping of blood cells in lymphoma and leukemia patients for making clinical diagnosis.